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Detection of Leptosphaeria Korrae, The Causal Agent of Necrotic Ring Spot, and its Occurrence in Southern Ontario

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dc.contributor.advisor Hsiang, Tom
dc.contributor.author O'Gorman, Daniel
dc.date.accessioned 2021-07-29T17:37:50Z
dc.date.available 2021-07-29T17:37:50Z
dc.date.copyright 1994
dc.date.created 1994-01
dc.date.issued 1994-01
dc.identifier.uri https://hdl.handle.net/10214/26146
dc.description.abstract Isolations from 122 suspected necrotic ring spot (NRS) samples collected throughout southern Ontario, were used to confirm the presence of the disease in the province. Based on morphology of the sexual structures, Leptosphaeria korrae, the causal agent of NRS, was confirmed in 17 Ontario counties. To increase the efficiency of pathogen detection, an assay based on the polymerase chain reaction (PCR), was tested using the primer pair, LK17S/5.8SC, selected from the internal transcribed spacer region 1 of L. korrae ribosomal DNA. Specific amplification of L. korrae DNA was obtained, while no amplification occurred with DNA isolated from 15 other fungal species or healthy Kentucky bluegrass, the major host of NRS. The use of PCR with RAPD (random amplified polymorphic DNA) primers, allowed detection of genetic variability in L. korrae single spore siblings and isolates collected from a single field site. The distribution pattern of polymorphic isolates at the field site suggests both mycelial growth and released ascospores were involved in the spread of NRS. en_US
dc.description.sponsorship Ontario Turfgrass Research Foundation, Ontario Ministry of Agriculture and Food en_US
dc.language.iso en en_US
dc.publisher University of Guelph en_US
dc.subject necrotic ring spot en_US
dc.subject plant pathology en_US
dc.subject turfgrass en_US
dc.subject pathogen detection en_US
dc.title Detection of Leptosphaeria Korrae, The Causal Agent of Necrotic Ring Spot, and its Occurrence in Southern Ontario en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Environmental Biology en_US
dc.rights.license All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.degree.grantor University of Guelph en_US


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