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Season and management related changes in the diversity of nitrifying and denitrifying bacteria in an Ontario agricultural soil

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Title: Season and management related changes in the diversity of nitrifying and denitrifying bacteria in an Ontario agricultural soil
Author: Smith, Jillian M.
Department: Department of Land Resource Science
Advisor: Dunfield, Kari E.
Abstract: This research assessed the effects of seasonal variation and tillage practice on the diversity of nitrous oxide producing bacteria in agricultural soil and investigated the dynamics of denitrifiers in a thawing soil in the laboratory. Diversity of the nitrifier and denitrifier communities was assessed by PCR-denaturing gradient gel electrophoresis (DGGE) using primer pairs targeting functional genes. Analysis revealed that seasonal variation had the largest influence on diversity; while tillage practices also influenced the diversity of the microbial communities. Diversity of the communities was the lowest in February, in frozen soils, and rapidly increased in March, after a spring thaw event. The laboratory study indicated that active denitrifiers were related to 'N'2'O' emission during this time, with increased diversity and richness associated with increased ' N'2'O'. Insight into the effects of tillage and seasonal variation on these microbial communities has the potential of improving our understanding of the mechanisms of 'N'2 'O' emissions.
URI: https://hdl.handle.net/10214/26092
Date: 2007
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