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Characterization of extended-spectrum cephalosporin-resistant Enterobacterales recovered from dairy manure in Southern Ontario

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dc.contributor.advisor Boerlin, Patrick
dc.contributor.author Anderson, Rebecca
dc.date.accessioned 2021-06-10T14:52:32Z
dc.date.available 2021-06-10T14:52:32Z
dc.date.copyright 2021-06
dc.date.created 2021-06-02
dc.identifier.uri https://hdl.handle.net/10214/25906
dc.description.abstract Few studies have investigated and characterized extended-spectrum cephalosporin (ESC) resistance among Enterobacterales recovered from dairy manure and even less research has been conducted on ESC-resistance plasmids among Escherichia coli and Klebsiella pneumoniae from livestock in Canada. The objectives of this research focused on the distribution and persistence of ESC-resistant E. coli and K. pneumoniae strains and their ESC-resistance determinants through manure treatment on dairy farms in Southern Ontario. Isolates were recovered from manure samples spanning treatment pipelines on six dairy farms. E. coli and K. pneumoniae carrying blaCTX-M and a subset of E. coli carrying blaCMY underwent whole genome sequencing (WGS) using Illumina short reads, with a subset of these isolates undergoing long read sequencing using Oxford Nanopore Technologies. Results suggest that E. coli is a driving force in the dissemination of ESC-resistance in Enterobacterales in dairy manure and K. pneumoniae also seems important but appears to be less prevalent. en_US
dc.description.sponsorship NSERC, CIHR, JPI-AMR, Ontario Veterinary College, Graduate Student Stipend Support Scholarship and the Queen Elizabeth II Graduate Student in Science and Technology Scholarship en_US
dc.language.iso en en_US
dc.publisher University of Guelph en_US
dc.subject dairy manure en_US
dc.subject anaerobic digestion en_US
dc.subject antimicrobial resistance en_US
dc.subject Enterobacterales en_US
dc.subject Canada en_US
dc.subject plasmids en_US
dc.subject extended-spectrum cephalosporins en_US
dc.subject CTX-M en_US
dc.subject CMY en_US
dc.title Characterization of extended-spectrum cephalosporin-resistant Enterobacterales recovered from dairy manure in Southern Ontario en_US
dc.type Thesis en_US
dc.degree.programme Pathobiology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Pathobiology en_US
dc.rights.license All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.degree.grantor University of Guelph en_US


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