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Preliminary Characterization of Vorin and VorinI: a Putative Toxin-Antitoxin Pair Produced by Erwinia Amylovora

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Title: Preliminary Characterization of Vorin and VorinI: a Putative Toxin-Antitoxin Pair Produced by Erwinia Amylovora
Author: Thow, Zachary
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Merrill, Rod
Abstract: Fire blight is a lethal disease of the Rosaceae plant family caused by Erwinia amylovora. A previous bioinformatics approach led to the discovery of Vorin and VorinI in E. amylovora as putative virulence factors of this plant pathogen. Vorin/VorinI is a novel type II toxin-antitoxin pair, where the effector, Vorin, is a secreted virulence factor and a mono-ADP-ribosyltransferase (mART) toxin that is toxic to both eukaryotes and bacteria. The non-lethal catalytic domain of Vorin, Vorin-cat, could not be expressed in E. coli despite reduced transcript levels, periplasmic expression, or semi-synthetic production. An inactive Vorin-cat variant was purified from inclusion bodies, successfully refolded, and was shown to bind NAD+ substrate. The immunity partner, VorinI was also purified and shown to be a properly folded protein. Further characterization of this novel toxin-antitoxin system may facilitate the development of anti-virulence compounds to treat fire blight in Rosaceae.
URI: https://hdl.handle.net/10214/25661
Date: 2021-04
Rights: Attribution-ShareAlike 4.0 International
Terms of Use: All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
Related Publications: Tremblay O, Thow Z, Geddes-McAlister J, Merrill AR. Several New Putative Bacterial ADP-Ribosyltransferase Toxins Are Revealed from In Silico Data Mining, Including the Novel Toxin Vorin, Encoded by the Fire Blight Pathogen Erwinia amylovora. Toxins. 2020; 12(12):792. https://doi.org/10.3390/toxins12120792


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Attribution-ShareAlike 4.0 International Except where otherwise noted, this item's license is described as Attribution-ShareAlike 4.0 International