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ABCNet: Predicting Chromosomal Compartments Directly from Reference Genomes

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Title: ABCNet: Predicting Chromosomal Compartments Directly from Reference Genomes
Author: Kirchhof, Matthew
Department: School of Computer Science
Program: Computer Science
Advisor: Kremer, Stefan
Abstract: Previous research has shown that the 3D organization of a cell’s genome is crucial to its functionality, directly contributing to gene regulation. Better understanding what gives rise to a genomes 3D organization, as well as the effects it has on a cell’s functionality, has the potential to lead to various breakthroughs surrounding disease, cell differentiation and more. In 2009, a biochemical assay used to capture the conformation of a genome (called Hi-C) was published, allowing us to better understand how a genome might interact with itself. From this, we can categorize regions of the genome into two compartments, those that are open (A) and those that are closed (B), where A compartments are gene rich, transcriptionally active and more loosely packed together than B compartments. This thesis presents ABCNet, a convolutional neural network (CNN) designed to predict the A/B compartments of a genome directly from its genomic sequence. Unlike other neural networks, ABCNet does not rely on a predetermined set of extracted genomic features and/or elements to make its predictions, while still matching their accuracy. Furthermore, analysis into ABCNet’s latent space hints at important genomic features indicative of its 3D genome organization.
URI: https://hdl.handle.net/10214/25225
Date: 2021-04
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