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Skeletal muscle metabolism is unaffected by DCA infusion and hyperoxic breathing at the onset of intense aerobic exercise

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Title: Skeletal muscle metabolism is unaffected by DCA infusion and hyperoxic breathing at the onset of intense aerobic exercise
Author: Savasi, Ingrid
Department: Department of Human Biology and Nutritional Sciences
Advisor: Spriet, Lawrence L.
Abstract: This study investigated whether increasing oxidative substrate supply, in the form of acetyl-CoA and oxygen, increases the rate of oxidative phosphorylation, and consequently reduces the reliance on substrate level phosphorylation, at the onset of intense aerobic exercise. Eight subjects cycled at 90% VO2max on three occasions, and received in randomized order: (1) normoxic breathing and saline infusion (control), (2) normoxic breathing and dichloroacetate infusion (increased acetyl-CoA), and (3) hyperoxic breathing (100% O2) and saline infusion (increased oxygen). Dichloroacetate significantly increased pyruvate dehydrogenase activation, acetyl-CoA, and acetylcarnitine contents at rest. However, similar levels of substrate level phosphorylation occurred in all trials, as reflected by phosphocreatine degradation and lactate accumulation. These data suggest that there is an inability to utilise extra oxidative substrate, implying that metabolic inertia at the level of pyruvate dehydrogenase and oxygen delivery are not limiting the rate of oxidative phosphorylation at the onset of intense aerobic exercise.
URI: https://hdl.handle.net/10214/25006
Date: 2000
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