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Induction of phenylalanine ammonia-lyase (PAL5) in tomato (Lycopersicon esculentum) during early Verticillium albo-atrum colonization

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dc.contributor.advisor Robb, E.J.
dc.contributor.author Saparno, Audrey M.
dc.date.accessioned 2021-04-19T14:27:04Z
dc.date.available 2021-04-19T14:27:04Z
dc.date.copyright 1997
dc.identifier.uri https://hdl.handle.net/10214/24998
dc.description.abstract The expression of the phenylalanine ammonia-lyase gene (PAL5) in tomato results from the differential utilization of two initiation sites. This thesis investigates whether a correlation exists between the differential expression of the two PAL5 transcripts and the plant's response to early colonization by Verticillium albo-atrum. Using an excised petiolar model system, expression in resistant and susceptible near-isolines of tomato was studied. Wounding alone resulted in an immediate but preferential transient induction for the short transcript. Though fungal colonization caused a similar response, the short transcript was induced even further relative to that seen with wounding; and this induction spanned a 24-hour period post infection. These trends occurred similarly in both isolines irrespective of the colonization pattern of V. albo-atrum. While PAL5 gene induction remains an important feature in the plant's response to stress, results indicate that the use of the two PAL5 initiation sites does not play a role in resistance of tomato to V. albo-atrum.
dc.language.iso en
dc.subject tomato
dc.subject phenylalanine ammonia-lyase
dc.subject verticillium albo-atrum
dc.subject early colonization
dc.subject stress
dc.title Induction of phenylalanine ammonia-lyase (PAL5) in tomato (Lycopersicon esculentum) during early Verticillium albo-atrum colonization
dc.type Thesis
dc.degree.name Master of Science
dc.degree.department Department of Molecular Biology and Genetics
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