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An analysis of alfalfa, Medicago sativa L., F1 families segregating for superoxide dismutase and alcohol dehydrogenase transgenes

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Title: An analysis of alfalfa, Medicago sativa L., F1 families segregating for superoxide dismutase and alcohol dehydrogenase transgenes
Author: Samis, Karen Elizabeth
Department: Department of Crop Science
Advisor: Bowley, Steve R.
Abstract: This thesis investigated the influence of multiple transgenes in an F1 alfalfa population on factors related to persistence. Parent genotypes contained either a TR'1-TR '2-regulated alcohol dehydrogenase ('Adh') gene or one of three 35S-regulated superoxide dismutase ('Sod') cDNAs: mitochondria-MnSOD, chloroplast-MnSOD or chloroplast-FeSOD. Families were characterized using Southern hybridization, PCR, SOD isozyme activity assays and biomass measurements from field trials. Transgenes followed the expected Mendelian segregation patterns in all F1 families except for one, which showed less 'Adh' transgene inheritance than expected. Novel SOD isozyme activity was detected in most genotypes with 'Sod' transgenes, although some forms of isozyme inactivity or transgene silencing were also detected. A synergistic transgene effect on SOD activity and on plant biomass was present in genotypes with both a 'Sod' transgene and an 'Adh' transgene. This research has provided the preliminary framework necessary for pyramiding multiple transgenes in alfalfa and for the genetic improvement of persistence in alfalfa.
URI: https://hdl.handle.net/10214/24988
Date: 2000
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