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Using bees to disseminate multiple fungal agents for insect pest control and plant disease suppression in greenhouse vegetables

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Title: Using bees to disseminate multiple fungal agents for insect pest control and plant disease suppression in greenhouse vegetables
Author: Shipp, Les; Kapongo, Jean Pierre; Kevan, Peter; Sutton, john; Broadbent, Bruce
Abstract: The bumble bee (Bombus impatiens) was used to vector a combined inoculum consisting of Beauveria bassiana (BotaniGard 22 WP) and Clonostachys rosea (ADJ 710 OMRI) in greenhouse tomato and sweet pepper for simultaneous control of insect pests (Lygus lineolaris and Trialeurodes vaporariorum) and plant disease suppression of grey mould. Four densities, 1) 6.24 x 1010 + 1.38 x 108; 2) 6.24 x 1010 +1.38 x 107; 3) 3.12 x1010 + 1.68 x 108; 4) 9.36 x 1010 + 1.06 x 108 conidia of B. bassiana and C. rosea respectively per g of inoculum and 5) a control (bees, but no inoculum) were assessed. Ninety-six, 47 and 88% of the bee, pest insect and plant samples contained detectable amounts of Beauveria and Clonostachys. Infection levels for both pest species, as well as, disease suppression were greatest at the highest concentrations of both agents. However, bee mortality was also significantly greater at the highest concentration of Beauveria. Therefore, the optimal combined concentration ratio of Beauveria and Clonostachys was 6.24 x 1010 B. bassiana + 1.38 x 107 C. rosea.
URI: http://hdl.handle.net/10214/2439
Date: 2008
Citation: Shipp, L., Kapongo, J.P., Kevan, P, Sutton, J., and Broadbent, B. "Using bees to disseminate multiple fungal agents for insect pest control and plant disease suppression in greenhouse vegetables" IOBC Bulletin(2008)


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