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Management of Plasmodiophora brassicae using fumigants and defense signalling

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Title: Management of Plasmodiophora brassicae using fumigants and defense signalling
Author: Robson, Justin
Department: Department of Plant Agriculture
Program: Plant Agriculture
Advisor: McDonald, Mary Ruth
Abstract: Clubroot (Plasmodiophora brassicae) is a soil-borne disease that attacks Brassica crops, including canola (Brassica napus). Management options are limited and the defense response in plants is not fully understood. Selected rates of the fumigants metam sodium and chloropicrin, along with solarization, were tested in controlled environment and fields studies. Two formulations of metam sodium performed similarly. Fumigants required sealing with high-grade film to be effective. Chloropicrin was more effective at reducing disease severity but neither fumigant eliminated resting spores from soil in trials. Solarization appeared to have promise, but requires more study. A split-root system for canola was developed to examine gene expression in roots challenged with P. brassicae. The splitting system prevented infection moving between root sections. Minimal testing was done with defense genes, but two defence genes, BnOPR2 and BnCCR, were consistently amplified. Results differed between the studies, which indicated that gene expression differed between the root sections.
URI: https://hdl.handle.net/10214/24086
Date: 2021
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