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A Digital Microfluidic Device for Antibiotic Testing in Milk

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Title: A Digital Microfluidic Device for Antibiotic Testing in Milk
Author: Eswar, Rahul
Department: School of Engineering
Program: Engineering
Advisor: Collier, ChristopherSingh, Ashutosh
Abstract: Worldwide, milk is tested to meet limits for antibiotic residue content which can invoke allergic reaction and antibiotic resistance in human consumers. Dairy farmers are responsible for collecting milk and shipping it to off-site laboratories for testing. Non-compliant milk results in penalties including fines and loss of licensure for offending dairy farmers. Modern on-site testers require long response times and manual preparation procedures which are unavailable during milk collection. An appealing technology to address this issue is digital microfluidics which handles sample in discrete droplets to leverage reconfigurability and automation. This thesis demonstrates the fundamental functionalities required by an on-site digital microfluidic dairy tester. Design of an integrated digital microfluidic device for milk droplet actuation is detailed. A label-free method for antibiotic detection in milk droplets is demonstrated using lock-in amplified fluorescence spectroscopy. This work provides the foundation for development of a full on-site digital microfluidic dairy testing solution.
Date: 2021-01
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