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Effect of Serum and Serum-Free Supplementation on Equine Mesenchymal Stromal Cells During In-Vitro Culture

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Title: Effect of Serum and Serum-Free Supplementation on Equine Mesenchymal Stromal Cells During In-Vitro Culture
Author: Oji, Saba
Department: Department of Biomedical Sciences
Program: Biomedical Sciences
Advisor: Madan, Pavneesh
Abstract: Mesenchymal Stromal Cells (MSCs) are multipotent stromal cells with a variety of applications. For an in-vitro culture of MSCs, a serum-containing medium is typically used for proper cell growth. However, the exact effect of the serum on these cells is not well understood. To combat this, metabolomics was used to distinguish the global metabolite changes during long-term culture of undifferentiated cord-blood (CB) derived MSCs in serum and their transition from serum to serum-free medium. In the long-term culture, only two metabolites were significantly different between the early and late passage with both groups expressing similar pathway enrichment analysis. However, in the serum versus serum-free group, there were 44 metabolites that were expressed differently with the serum-free group lacking the Taurine and Hypo-taurine Metabolism pathway as compared to the serum group. Therefore, suggesting that both the passage number and the media condition could affect the growth of the equine CB-MSCs.
URI: https://hdl.handle.net/10214/23702
Date: 2021-01
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