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Protein Binding Partners of Glutathione Transferase Theta 1 (GSTT1)

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Title: Protein Binding Partners of Glutathione Transferase Theta 1 (GSTT1)
Author: Hoff, Amy
Department: Department of Biomedical Sciences
Program: Molecular and Cellular Biology
Advisor: Josephy, DavidCoppolino, Marc
Abstract: Glutathione transferase (GST) enzymes are found in all kingdoms of life. Their catalytic activity is well characterized: conjugation of glutathione with electrophiles, a process which inactivates many toxic chemicals. Additionally, several GST enzymes participate in cell signaling processes, mediated by protein binding interactions. This thesis focuses on human GST theta 1. 30-40% of the human population is homozygous null for the GSTT1 gene, but the consequences of this polymorphism remain unclear. I have searched for possible protein binding partners of GSTT1 through a pull-down assay with protein lysates from cultured human prostate cancer cells and erythrocytes, followed by mass spectrometry-based proteomic analysis. Histidine Triad Nucleotide Binding Protein-1 (HINT1) was identified as a binding partner. HINT1, a member of the HIT superfamily of nucleotide hydrolases and transferases; is also known to be a tumor suppressor. An interaction between GSTT1 and HINT1 may point towards a previously unknown biological role of GSTT1.
URI: https://hdl.handle.net/10214/23683
Date: 2020
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
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Hoff_Amy_202012_MSc.pdf 922.3Kb PDF View/Open Thesis
Hoff_Amy_202012_MSc_APP_B_PC-3pull-down1.csv 392.1Kb Unknown View/Open Data set 1
Hoff_Amy_202012_MSc_APP_B_PC-3pull-down2.csv 369.7Kb Unknown View/Open Data set 2
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Hoff_Amy_202012_MSc_APP_B_RBCpull-down1-2.csv 89.52Kb Unknown View/Open Data set 5
Hoff_Amy_202012_MSc_APP_B_PC-3pull-down4.csv 1.397Mb Unknown View/Open Data set 6

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Attribution-NonCommercial-NoDerivatives 4.0 International Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International