Title:
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Bacteroides genetic markers for microbial source tracking fecal pollution in Ontario waters |
Author:
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Avedesian, Jeffrey V
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Department:
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School of Environmental Sciences |
Program:
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Environmental Sciences |
Advisor:
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Habash, Marc B |
Abstract:
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This thesis investigated the laboratory and field validation of a Bacteroides swine marker and developed a Bacteroides chicken marker for microbial source tracking (MST) in Ontario watersheds. The BacSwine genetic marker was validated in the laboratory using 146 fecal samples from target and non-target species. The marker produced a binary sensitivity of 1.0 and a specificity of 0.79 with statistical reasoning to distinguish between true and false positives with > 98% confidence. The BacChicken marker was tested for presence/absence on 35 target and non-target species and produced a sensitivity of 0.92 and specificity of 0.96. The MST BacGeneral, BacBovine, BacHuman, and BacSwine markers were applied in parallel with E. coli at point and non-point locations in the Grand River and a small inland lake watershed. Seasonal changes, land use practices, and land development were likely related to changes in dominant marker abundance specific to sample locations. |
URI:
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https://hdl.handle.net/10214/23673
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Date:
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2020-11 |
Rights:
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Attribution-NoDerivatives 4.0 International |
Terms of Use:
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