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Bacteroides genetic markers for microbial source tracking fecal pollution in Ontario waters

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Title: Bacteroides genetic markers for microbial source tracking fecal pollution in Ontario waters
Author: Avedesian, Jeffrey V
Department: School of Environmental Sciences
Program: Environmental Sciences
Advisor: Habash, Marc B
Abstract: This thesis investigated the laboratory and field validation of a Bacteroides swine marker and developed a Bacteroides chicken marker for microbial source tracking (MST) in Ontario watersheds. The BacSwine genetic marker was validated in the laboratory using 146 fecal samples from target and non-target species. The marker produced a binary sensitivity of 1.0 and a specificity of 0.79 with statistical reasoning to distinguish between true and false positives with > 98% confidence. The BacChicken marker was tested for presence/absence on 35 target and non-target species and produced a sensitivity of 0.92 and specificity of 0.96. The MST BacGeneral, BacBovine, BacHuman, and BacSwine markers were applied in parallel with E. coli at point and non-point locations in the Grand River and a small inland lake watershed. Seasonal changes, land use practices, and land development were likely related to changes in dominant marker abundance specific to sample locations.
URI: https://hdl.handle.net/10214/23673
Date: 2020-11
Rights: Attribution-NoDerivatives 4.0 International


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Attribution-NoDerivatives 4.0 International Except where otherwise noted, this item's license is described as Attribution-NoDerivatives 4.0 International