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Gas-Phase Ion Chemistry Studies Combined with Differential Ion Mobility for Separating, Detecting, and Identifying Protomers of Guanine by Mass Spectrometry

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Title: Gas-Phase Ion Chemistry Studies Combined with Differential Ion Mobility for Separating, Detecting, and Identifying Protomers of Guanine by Mass Spectrometry
Author: Yee, Allyson
Department: Department of Chemistry
Program: Chemistry
Advisor: Gabryelski, Wojciech
Abstract: Protomers of guanine and its derivatives can be partially separated by FAIMS and each protomer exhibits unique CID pathways associated with its protonation site. This is the first successful attempt to generate, identify, and detect guanine protomers. Compensation voltage (CV) spectra of guanine and its derivatives are problematic in the separation of protomers by FAIMS. The addition of carbon dioxide in the carrier gas improves the separation of protomers in FAIMS, protecting them from potential proton exchanges before detection. Collision induced dissociation (CID) under soft fragmentation conditions allow for the identification of protomers based off of the fragmentation pathway. Despite being unable to fully separate the protomers, we were able to recognize and correlate CID products of each protomer to its initial charge site based on the CV spectra. This type of analysis has never been done before in mass spectrometry. The identification of protomers by FAIMS and their further analysis in tandem mass spectrometry create new capabilities in mass spectrometry analysis of small molecules. This new method provides a very important tool for studying gas phase ion chemistry and for practical application such as identification of small molecules using tandem mass spectrometry.
URI: https://hdl.handle.net/10214/23522
Date: 2020-12
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