Main content

Design, development and testing of a system to measure sarcomere length by laser diffraction in working muscle in vitro

Show simple item record

dc.contributor.advisor Stevens, E.D.
dc.contributor.author Freeman, Marcus
dc.date.accessioned 2020-12-03T14:30:05Z
dc.date.available 2020-12-03T14:30:05Z
dc.date.copyright 2000
dc.identifier.uri https://hdl.handle.net/10214/21504
dc.description.abstract The purpose of this thesis was to design, construct and test a system to measure sarcomere length by loser diffraction in striated muscle ' in vitro'. I designed a muscle chamber to allow illumination of all points on a muscle, transmission of the diffracted light, temperature control and stimulation. Control software was developed to detect the position of diffracted orders. Force, muscle length and sarcomere length data were integrated. To test the system, force, muscle length and sarcomere length were measured in frog semitendinosus muscles during passive stretch, fixed-length tetanic (isometric) contractions, and contractions during sinusoidal muscle length changes (eccentric). Sarcomere length changed linearly with muscle length during passive stretches from 2.3 to 2.7 [mu]m while force increased curvilinearly to 0.14 mN. In isometric and eccentric contractions sarcomere length shortened from 2.6 to 2.3 [mu]m, but force was higher in the eccentric (0.14 mN) than in the isometric contractions (0.12 mN). en_US
dc.language.iso en
dc.publisher University of Guelph en_US
dc.subject measurement system en_US
dc.subject sarcomere length en_US
dc.subject loser diffraction en_US
dc.subject striated muscle en_US
dc.subject in vitro en_US
dc.subject muscle chamber en_US
dc.subject control software en_US
dc.subject force en_US
dc.subject muscle length en_US
dc.title Design, development and testing of a system to measure sarcomere length by laser diffraction in working muscle in vitro en_US
dc.type Thesis en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Zoology en_US
dc.rights.license All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.degree.grantor University of Guelph en_US


Files in this item

Files Size Format View
Freeman_Marcus_MSc.pdf 2.653Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record