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Quantitative PCR Detection of Tenacibaculum Maritimum and Tenacibaculum Dicentrarchi at Netpen Sites in British Columbia (Canada)

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dc.contributor.advisor Lumsden, John
dc.contributor.advisor Russell, Spencer
dc.contributor.author Nowlan, Joseph
dc.date.accessioned 2020-09-16T21:12:33Z
dc.date.available 2020-09-16T21:12:33Z
dc.date.copyright 2020-09
dc.date.created 2020-09-10
dc.identifier.uri https://hdl.handle.net/10214/21292
dc.description.abstract Several Tenacibaculum bacterial species are detrimental to aquaculture, inducing tenacibaculosis in fishes, but information on the bacteria’s ecology is limited. Two quantitative-PCR assays for T. maritimum and T. dicentrarchi were used to survey two separate Atlantic salmon netpen sites in 2019-20 in British Columbia. Approximately 100-fold more T. dicentrarchi were recorded compared to T. maritimum (p<4e-7). During outbreaks, bacteria were ubiquitous with greater numbers in eukaryotes (p<8e-13). Significantly (p<0.05) more bacteria were identified during with no difference after an outbreak, supporting the observed recrudescence of tenacibaculosis. There was a significant relationship (p<0.05) between the water quality and fish mortalities, but with low correlation coefficients (R2<0.25). High numbers of T. dicentrarchi were present at both netpen sites, increased T. dicentrarchi was detected concurrent with antibiotic treatments and also in dead fish tissues relative to live tissues. T. dicentrarchi seems most responsible for the disease outbreaks observed at both netpen sites. en_US
dc.description.sponsorship NSERC Engage and Engage Plus Grant VIU RIF Grant en_US
dc.language.iso en en_US
dc.publisher University of Guelph en_US
dc.rights Attribution-NoDerivatives 4.0 International *
dc.rights.uri https://creativecommons.org/licenses/by-nd/4.0/ *
dc.subject Fish en_US
dc.subject Bacteria en_US
dc.subject Tenacibaculum en_US
dc.subject qPCR en_US
dc.subject Pathobiology en_US
dc.title Quantitative PCR Detection of Tenacibaculum Maritimum and Tenacibaculum Dicentrarchi at Netpen Sites in British Columbia (Canada) en_US
dc.type Thesis en_US
dc.degree.programme Pathobiology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Pathobiology en_US
dc.degree.grantor University of Guelph en_US


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Attribution-NoDerivatives 4.0 International Except where otherwise noted, this item's license is described as Attribution-NoDerivatives 4.0 International