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Improving traceability and detection of food fraud: An exploration of current seafood authentication methods and validation of a novel qPCR and closed-tube barcoding method for commercial species of fish, FASTFISH-ID

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Title: Improving traceability and detection of food fraud: An exploration of current seafood authentication methods and validation of a novel qPCR and closed-tube barcoding method for commercial species of fish, FASTFISH-ID
Author: Singh, Maleeka
Department: Department of Food Science
Program: Food Science
Advisor: Farber, JeffreyHanner, Robert
Abstract: Food fraud is a global issue, which not only defrauds the consumer, but presents a food safety risk. Seafood is one of the most adulterated foods due to the vast and complex supply chains. Over the past 20 years, 236 original DNA-based assays have been published for the authentication of commercial species of fish. qPCR has been the most researched DNA-based authentication method, followed by DNA sequencing. The target genes cytb (cytochrome b) and COI (cytochrome c oxidase 1) are also the most researched in the literature. However, in silico testing on a select set of published assays showed a lack of assay reliability. A total of 77.78% of the tested assays were found to be non-specific. A novel DNA-based authentication method, using qPCR and closed-tube barcoding technology, called FASTFISH-ID, can provide a quick and reliable, first-screening step, in fish authentication.
URI: https://hdl.handle.net/10214/21177
Date: 2020-09
Rights: Attribution 4.0 International
Terms of Use: All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.


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Attribution 4.0 International Except where otherwise noted, this item's license is described as Attribution 4.0 International