Main content

Comparing the Potency, Stability and Cell Stimulating Activity of Protegrin-1 with a Hybrid Antimicrobial Peptide

Show full item record

Title: Comparing the Potency, Stability and Cell Stimulating Activity of Protegrin-1 with a Hybrid Antimicrobial Peptide
Author: Maystrenko, Anna
Department: Department of Animal Biosciences
Program: Animal and Poultry Science
Advisor: Li, Julang
Abstract: Multi-drug resistant (MDR) bacteria and their biofilms are a concern in veterinary and human medicine. Protegrin-1 (PG-1) is a potent antimicrobial peptide (AMP) with immunomodulatory properties, but its application as a therapeutic is limited by its instability in physiological conditions. To improve stability of PG-1, a hybrid peptide, SynPG-1, was designed. The aim of this study was to compare these two peptides in three categories: antimicrobial and antibiofilm activity against MDR pathogens; stability within physiological fluids; and impact on IPEC-J2 cell line. The results show that SynPG-1 demonstrated a 12-fold increase in serum stability from PG-1 and retained activity after 24hrs of pre-incubation. Both peptides marginally inhibited formation of biofilms and reduction of biomass in pre-formed biofilms, but showed substantial reduction in bacteria within biofilms. Peptides maintained activity after incubation with trypsin and porcine gastric fluid, but not intestinal fluid, and were able to stimulate IPEC-J2 cell migration.
URI: https://hdl.handle.net/10214/21163
Date: 2020-08
Rights: Attribution 4.0 International
Terms of Use: All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
Related Publications: Maystrenko, A.; Feng, Y.; Akhtar, N.; Li, J. The Addition of a Synthetic LPS-Targeting Domain Improves Serum Stability While Maintaining Antimicrobial, Antibiofilm, and Cell Stimulating Properties of an Antimicrobial Peptide. Biomolecules 2020, 10, 1014. https://doi.org/10.3390/biom10071014


Files in this item

Files Size Format View Description
Maystrenko_Anna_202008_MSc.pdf 1.318Mb PDF View/Open Anna Maystrenko's MSc Thesis

This item appears in the following Collection(s)

Show full item record

Attribution 4.0 International Except where otherwise noted, this item's license is described as Attribution 4.0 International