Main content

Evaluating the Clearance of Common Extra-label Drug Combinations by Turkey Hepatocytes in Primary Culture

Show full item record

Title: Evaluating the Clearance of Common Extra-label Drug Combinations by Turkey Hepatocytes in Primary Culture
Author: Lyster, Samantha
Department: Department of Biomedical Sciences
Program: Biomedical Sciences
Advisor: Johnson, RonKirby, Gordon M.
Abstract: Although common in the commercial turkey industry, concurrent use of two or more drugs in the same feed can result in drug-drug interactions that may impact drug clearance. Therefore, this thesis tested the hypothesis that primary turkey hepatocyte cultures are useful as a high-throughput in vitro model to screen for drug-drug interactions with drug combinations in poultry feed. The cultures were validated by confirming the presence of proteins, enzymes, and enzyme processes that are involved in drug clearance. The in vitro model was successfully used to monitor the clearance of fenbendazole and monensin and to assess differences in the rates of clearance when the drugs were used alone versus in combination. Linear regression analysis revealed that the rate of clearance of fenbendazole in combination was 13.3% slower than fenbendazole clearance on its own. Additional experiments are needed to determine the clinical and human food safety relevance of the observed interaction.
URI: https://hdl.handle.net/10214/20732
Date: 2020-08
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
Terms of Use: All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.


Files in this item

Files Size Format View
Lyster_Samantha_202008_MSc.pdf 1.399Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record

Attribution-NonCommercial-NoDerivatives 4.0 International Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International