Main content

Antisense oligodeoxynucleotide-mediated alterations in slow troponin C gene expression during myogenesis

Show full item record

Title: Antisense oligodeoxynucleotide-mediated alterations in slow troponin C gene expression during myogenesis
Author: Choudhury, Monideepa
Department: Department of Molecular Biology and Genetics
Advisor: Bag, J.
Abstract: The expression of genes for contractile proteins during terminal differentiation in myogenesis is coordinately regulated. It remains unclear how the transcriptional and translational machinery in the cell is fine tuned to achieve the proper stoichiometric representation of each polypeptide complement of the multicomponent myofilament. To help elucidate the complex regulatory mechanisms that govern myogenesis, the expression of the slow troponin C (s/c TnC) gene in cell culture as a model system has been studied. The effect on myogenesis, of specifically blocking the expression of the s/cTnC gene with an antisense phosphorothioate (PS) oligodeoxynucleotide (ODN) complexed with the DOSPER liposome (Boehringer Mannheim) was observed. A 4 day treatment period with 1 $\mu$M of the antisense ODN in 1 ml differentiation medium resulted in approximately a 80% and 50% decrease in the s/cTnC polypeptide synthesis and mRNA levels. However, a corresponding reduction of the steady state level of this polypeptide was not observed. There was also no appreciable alteration in the pattern of myogenesis by ODN treatment as judged by the synthesis of other muscle specific proteins. Furthermore, the antisense effect was found to be transient as the cells recovered to synthesize normal levels of s/c TnC polypeptide upon withdrawal of the antisense ODN from the culture medium. The results presented in this thesis suggests $\rm C\sb2C\sb{12}$ myotubes possesses an internal compensatory mechanism(s) that attempts to overcome the block on the expression of the s/cTnC gene to maintain the desired stoichiometry in the formation of a functional contractile apparatus.
Date: 1997
Terms of Use: All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.

Files in this item

Files Size Format View
Choudhury_Monideepa_MSc.pdf 6.207Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record

The library is committed to ensuring that members of our user community with disabilities have equal access to our services and resources and that their dignity and independence is always respected. If you encounter a barrier and/or need an alternate format, please fill out our Library Print and Multimedia Alternate-Format Request Form. Contact us if you’d like to provide feedback:  (email address)