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Improving Lyme Disease Direct Detection: Identification of a Novel Method to Enrich for Borrelia in Blood, and Comparing Surveys and Serology in a Chronically Ill Patient Cohort

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dc.contributor.advisor Khursigara, Cezar
dc.contributor.advisor Wills, Melanie
dc.contributor.author Sanderson, Victoria
dc.date.accessioned 2020-06-16T20:45:43Z
dc.date.copyright 2020-06-02
dc.date.created 2020-06-02
dc.date.issued 2020-06-16
dc.identifier.uri http://hdl.handle.net/10214/18041
dc.description.abstract Lyme disease is a multi-system disease caused by the spirochetal bacterium, Borrelia, an obligate parasite transmitted to humans by Ixodes ticks. The laboratory test for Lyme is reliant on the adaptive immune response which limits early diagnosis and is unable to distinguish between active and resolved infection. There is interest in tools that directly detect the microorganism and its associated biomarkers. However, there is no consensus regarding the methodologies used to process blood for this purpose. Our findings show sodium citrate anticoagulant is superior to commonly used EDTA for direct Borrelia detection in blood. Additionally, a mock-infected blood model demonstrates that serum and plasma lose the majority of Borrelia during processing, while the platelet fraction is enriched. Serostatus and surveys were analyzed for a 70-participant cohort of chronically ill patients and healthy controls to compare existing diagnostic techniques. This research provides foundational work informing future avenues of direct detection research. en_US
dc.description.sponsorship G. Magnotta Foundation for Vector Bourne Disease en_US
dc.language.iso en en_US
dc.subject Borrelia en_US
dc.subject Lyme disease en_US
dc.subject chronic Lyme disease en_US
dc.subject post-treatment Lyme disease syndrome en_US
dc.subject platelet en_US
dc.subject blood en_US
dc.subject diagnostic en_US
dc.title Improving Lyme Disease Direct Detection: Identification of a Novel Method to Enrich for Borrelia in Blood, and Comparing Surveys and Serology in a Chronically Ill Patient Cohort en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US
dc.description.embargo 2021-06-02
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