dc.contributor.advisor |
Guzman, Ernesto |
|
dc.contributor.advisor |
Merrill, Rod |
|
dc.contributor.author |
Turner, Madison
|
|
dc.date.accessioned |
2020-05-25T17:06:22Z |
|
dc.date.available |
2020-05-25T17:06:22Z |
|
dc.date.copyright |
2020-05 |
|
dc.date.created |
2020-05-14 |
|
dc.date.issued |
2020-05-25 |
|
dc.identifier.uri |
http://hdl.handle.net/10214/17965 |
|
dc.description.abstract |
Apis mellifera populations are declining due to a variety of factors, including the parasitic mite, Varroa destructor, and the bacterial disease, American foulbrood (AFB). This thesis describes the biochemical characterization of two important virulence factors: LarvinA from AFB, and Varroa Toxic Protein (VTP) from the mite. LarvinA was kinetically characterized and confirmed to be a functional C3-like mono-ADP-ribosyltransferase toxin. N-terminal deletions and single-residue variants led to the identification of novel interactions between the α1-helix and the active-site of LarvinA, including the role of net charge in cell entry. Next, VTP was purified using glutathione-based affinity and size-exclusion chromatography. Analysis of the primary sequence and circular dichroism spectra revealed that VTP is primarily an α-helical protein; however, it is thermally labile based on the temperature analysis of the protein. These findings can be combined with future studies to elucidate novel therapeutics. |
en_US |
dc.language.iso |
en |
en_US |
dc.subject |
Apis mellifera |
en_US |
dc.subject |
Varroa |
en_US |
dc.subject |
American foulbrood |
en_US |
dc.subject |
Varroa Toxic Protein |
en_US |
dc.subject |
LarvinA |
en_US |
dc.title |
Characterization of virulence factors targeting Apis mellifera: Varroa Toxic Protein and LarvinA |
en_US |
dc.type |
Thesis |
en_US |
dc.degree.programme |
Molecular and Cellular Biology |
en_US |
dc.degree.name |
Master of Science |
en_US |
dc.degree.department |
Department of Molecular and Cellular Biology |
en_US |
dc.rights.license |
All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated. |
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