Main content

A platform upgrade will be performed on the Atrium Institutional Repository from Monday, July 13 to Wenesday, July 15, 2020 (inclusive). During this time, users will not be able to submit new items to the Atrium. Users will still be able to browse, view, and download items that are already available in the Atrium. We apologize for any inconvenience this may cause.

The heterogeneous nuclear ribonucleoprotein K is responsible for regulating the fate decisions of neural precursor cells of the developing cerebral cortex

Show full item record

Title: The heterogeneous nuclear ribonucleoprotein K is responsible for regulating the fate decisions of neural precursor cells of the developing cerebral cortex
Author: Brott, Julia
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Vessey, John
Abstract: Neurogenesis within the developing cortex occurs as a result of the controlled differentiation of neural precursor cells (NPCs), achieved by tightly regulated gene expression. Many RNA-binding proteins have been implicated in regulating gene expression throughout development, including the heterogeneous nuclear ribonucleoproteins (hnRNPs). Specifically, mutation of hnRNP-K has been identified in Kabuki-like syndrome, a neurodevelopmental disorder presenting with cortical atrophy, ventriculomegaly, and periventricular nodular heterotopia. This research validates hnRNP-K expression throughout the neurogenic period of cortical development and demonstrates a critical role for hnRNP-K in the cell fate decisions of NPCs. Knockdown of hnRNP-K in vitro resulted in a significant increase in the proportion of β3T+ neurons and a significant decrease in the proportion of Nestin+ NPCs. Immunoprecipitation revealed hnRNP-K maintains RNA-based interactions with proteins known to regulate gene expression, proliferation and differentiation. Collectively, these findings demonstrate a significant role for hnRNP-K in maintenance of NPC phenotype during murine cortical development.
URI: http://hdl.handle.net/10214/17886
Date: 2020-04
Terms of Use: All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.


Files in this item

Files Size Format View Description
Brott_Julia_202004_Msc.pdfuntranslated 2.160Mb PDF View/Open Thesis

This item appears in the following Collection(s)

Show full item record