Main content

Insertional vs Targeted Mutagenesis in the Development of Zebrafish as an In Vivo Model for Cardiomyopathy

Show full item record

Title: Insertional vs Targeted Mutagenesis in the Development of Zebrafish as an In Vivo Model for Cardiomyopathy
Author: Ojehomon, Matiyo
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Dawson, John
Abstract: Heart failure is a global economic burden and can be caused by cardiomyopathy, a disease of the myocardium. Mutations in genes encoding sarcomere proteins have been known to cause cardiomyopathy. One of these sarcomere proteins is α-cardiac actin (ACTC), which is needed for proper contraction of the heart. To better understand how mutations in the ACTC protein cause cardiomyopathy, an in vivo model like zebrafish can be used to understand the molecular mechanism that occurs. Using transposons (insertional) or Clustered Regularly Interspaced Palindromic Repeats (CRISPRs) (targeted), zebrafish can be engineered to carry these mutations and the impact can be studied. The transposon system proved to be unstable as the inserted transposon underwent transcriptional repression. To use the CRISPR system, identifying which zfactc gene to target is necessary. Performing WMISH and RT-qPCR, zfacta1b seems to be the best candidate for targeted mutagenesis due to its early expression in the heart.
URI: http://hdl.handle.net/10214/17885
Date: 2020-04-20
Rights: Attribution 4.0 International
Terms of Use: All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.


Files in this item

Files Size Format View
Ojehomon_Matiyo_202004_MSc.pdf 2.018Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record

Attribution 4.0 International Except where otherwise noted, this item's license is described as Attribution 4.0 International