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Optimization and validation of the extraction, purification and analysis of galacto-oligosaccharides (GOS) in beans

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dc.contributor.advisor Martinez Martinez, Mario Widjaja, Listiya 2020-04-23T18:21:45Z 2020-03-24 2020-03-24 2020-04-23
dc.description.abstract Pulses are rich in protein, dietary fiber, minerals and vitamins and their consumption promotes beneficial metabolic responses. However, postprandial gastrointestinal discomfort decreases the overall interest for pulses. Side effects are mostly attributed to Galacto-oligosaccharides (GOS), a group of indigestible carbohydrates formed by galactose monomers linked to a sucrose molecule by α (1→6) glycosidic linkage. Reducing these oligosaccharides is of interest to enhance pulse consumption; nevertheless, a reliable and reproducible extraction and analysis method needs to be developed. In this work, we optimized and validated the extraction and purification of GOS in red kidney bean (Phaseolus vulgaris), and the subsequent quantification through high-performance anion-exchange chromatography (HPAEC-PAD) and gas chromatography/mass spectrometry (GC/MS). With an optimized derivatization (to make them volatile) procedure, the GC/MS was more reproducible and reliable to identify and quantify the GOS content in 6 native bean kernels and canned whole beans exposed to different processing conditions. en_US
dc.description.sponsorship NSERC en_US
dc.language.iso en en_US
dc.subject galacto-oligosaccharides, beans, pulses, method optimization and validation, High-performance anionic exchange column with pulsed amperometric detection(HPAEC-PAD), Gas-chromatography with Mass spectrometry (GC-MS), derivatization, oximation, silylation, matrix-enhancement effect, response factor en_US
dc.subject alpha-1-6 glycosidic bond, FODMAPs, Irritable Bowel Syndrome (IBS), Linearity, accuracy, recovery percentage, matrix effect, precision, interday repeatabilty, intraday repeatability, GOS quantification, black turtle beans, romano beans, yellow eye beans, pinto beans, white navy beans, dark red kidney beans en_US
dc.subject protein, fiber, bean flour en_US
dc.title Optimization and validation of the extraction, purification and analysis of galacto-oligosaccharides (GOS) in beans en_US
dc.type Thesis en_US Engineering en_US Master of Applied Science en_US School of Engineering en_US
dc.description.embargo 2021-01-01
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