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Relevance of in vitro agar based screens to characterize the anti-fungal activities of bacterial endophyte communities

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Title: Relevance of in vitro agar based screens to characterize the anti-fungal activities of bacterial endophyte communities
Author: Shehata, Hanan; Lyons, Eric M.; Jordan, Katerina S.; Raizada, Manish N.
Department: Department of Plant Agriculture
Abstract: Background:Endophytes are microbes that inhabit internal plant tissues without causing disease. Plant microbialcommunities consist of large numbers of endophyte species. Understanding the functions of these endophytes is amajor challenge. An important function of some endophytes is to suppress fungal pathogens. Typically, plantassociated microbes are screened for anti-fungal activities in vitro using the high-throughput dual culture screen,but it is not clear whether this method correlates with the activities of these microbesin planta. Furthermore, it isnot clear whether in vitro screening captures all of the microbes that show this activity inside plants. The objectiveof this study was to evaluate the relevance of the in vitro dual culture method for screening endophytes withanti-fungal activity.Results:In parallel, 190 bacterial endophytes from the corn grass family (Zea) were screened for suppression oftwo fungal pathogens (Sclerotinia homoeocarpaandRhizoctonia solani) using the in vitro dual culture method,andin plantausing the model plant, creeping bentgrass. All endophytes that showed anti-fungal activityin plantaagainstSclerotinia homoeocarpa and Rhizoctonia solani(3 or 4 strains, respectively, out of 190), were captured in vitro.The in vitro andin plantascreening results strongly correlated (r = 0.81 and r = 0.94 for the two pathogens, respectively).Conclusions:Evidence was gained here that the in vitro dual culture method is a relevant method for high throughputscreening of plant endophyte communities for anti-fungal activity. In our study, the method captured all of the microbesthat suppressed the corresponding pathogensin planta.
URI: http://hdl.handle.net/10214/17857
Date: 2016
Rights: Attribution 4.0 International
Terms of Use: All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
Related Publications: Shehata, H.R., Lyons, E.M., Jordan, K.S. et al. Relevance of in vitro agar based screens to characterize the anti-fungal activities of bacterial endophyte communities. BMC Microbiol 16, 8 (2016). https://doi.org/10.1186/s12866-016-0623-9


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