Main content

Examining the Intraspecific Interactions in Mixed-Strain Biofilms Containing the Prairie Epidemic Strain and Unique Clinical Pseudomonas aeruginosa Isolates

Show simple item record

dc.contributor.advisor Khursigara, Cezar
dc.contributor.author Garnier, Nicole
dc.date.accessioned 2020-04-02T19:22:39Z
dc.date.available 2020-04-02T19:22:39Z
dc.date.copyright 2020-03
dc.date.created 2020-03-12
dc.date.issued 2020-04-02
dc.identifier.uri http://hdl.handle.net/10214/17839
dc.description.abstract The prairie epidemic strain (PES) of Pseudomonas aeruginosa causes infections in cystic fibrosis (CF) patients that lead to an increase in morbidity and mortality. The PES can cause superinfections and outcompete previously existing P. aeruginosa isolates within the lungs of CF patients, however, the interactions that occur to allow this to happen are currently unknown. This work examines biofilms formed when PES isolates are cocultured with clinical P. aeruginosa isolates that either remained stable (P637) or were replaced by the PES during a superinfection (P261). Biofilms were quantified using a crystal violet assay, imaged using fluorescence microscopy, and the isolates within the coculture biofilms were quantified using Droplet Digital™ PCR. The results of this project combined with recent sequence data suggests that there may be alterations in the regulation of biofilm formation in these cocultures, potentially due to changes in bacterial quorum sensing. en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject Pseudomonas en_US
dc.subject aeruginosa en_US
dc.subject epidemic strain en_US
dc.subject Prairie epidemic strain en_US
dc.subject PES en_US
dc.subject biofilm en_US
dc.subject microscopy en_US
dc.subject ddPCR en_US
dc.subject Droplet Digital PCR en_US
dc.subject P. aeruginosa en_US
dc.subject intraspecific interactions en_US
dc.subject coculture en_US
dc.title Examining the Intraspecific Interactions in Mixed-Strain Biofilms Containing the Prairie Epidemic Strain and Unique Clinical Pseudomonas aeruginosa Isolates en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US
dc.rights.license All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.


Files in this item

Files Size Format View
Garnier_Nicole_202003_Msc.pdf 2.459Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivatives 4.0 International Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International