Main content

Genomic Analysis of Canine Appendicular Osteosarcoma Metastases

Show simple item record

dc.contributor.advisor Wood, Geoffrey Szlachtycz, David 2020-01-10T17:19:23Z 2020-01 2019-12-09 2020-01-10
dc.description.abstract In this study, analysis was done on matched normal, primary, and metastatic samples of canine appendicular osteosarcoma that were preserved by either formalin fixation or freezing. The frozen samples were analyzed for copy number aberrations, RNA expression, and somatic variants, using arrayCGH, RNA microarrays, and DNA sequencing data. Comparison of DNA ploidy and RNA expression between matched primary and metastatic samples showed few significant changes suggesting that most changes happen before metastasis. Matched primary and metastatic samples from the same patient shared more somatic variants with each other than with samples from the same group. The frozen and formalin fixed samples were compared to analyze the artifacts caused by formalin fixation. The results showed that formalin fixed samples had lower quality properties than their frozen counterparts. Despite this, the formalin fixed samples still retained many of the biological patterns present in their frozen counterparts. en_US
dc.description.sponsorship Pet Trust, OVC Student Scholarship en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial 4.0 International *
dc.rights.uri *
dc.subject pathobiology canine osteosarcoma appendicular en_US
dc.title Genomic Analysis of Canine Appendicular Osteosarcoma Metastases en_US
dc.type Thesis en_US Pathobiology en_US Master of Science en_US Department of Pathobiology en_US
dc.description.embargo 2020-12-09
dc.rights.license All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.

Files in this item

Files Size Format View Description
Szlachtycz_David_202001_Msc.pdfuntranslated 2.761Mb PDF View/Open thesis

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial 4.0 International Except where otherwise noted, this item's license is described as Attribution-NonCommercial 4.0 International