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Genomic Analysis of Canine Appendicular Osteosarcoma Metastases

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dc.contributor.advisor Wood, Geoffrey
dc.contributor.author Szlachtycz, David
dc.date.accessioned 2020-01-10T17:19:23Z
dc.date.copyright 2020-01
dc.date.created 2019-12-09
dc.date.issued 2020-01-10
dc.identifier.uri http://hdl.handle.net/10214/17768
dc.description.abstract In this study, analysis was done on matched normal, primary, and metastatic samples of canine appendicular osteosarcoma that were preserved by either formalin fixation or freezing. The frozen samples were analyzed for copy number aberrations, RNA expression, and somatic variants, using arrayCGH, RNA microarrays, and DNA sequencing data. Comparison of DNA ploidy and RNA expression between matched primary and metastatic samples showed few significant changes suggesting that most changes happen before metastasis. Matched primary and metastatic samples from the same patient shared more somatic variants with each other than with samples from the same group. The frozen and formalin fixed samples were compared to analyze the artifacts caused by formalin fixation. The results showed that formalin fixed samples had lower quality properties than their frozen counterparts. Despite this, the formalin fixed samples still retained many of the biological patterns present in their frozen counterparts. en_US
dc.description.sponsorship Pet Trust, OVC Student Scholarship en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial 4.0 International *
dc.rights.uri http://creativecommons.org/licenses/by-nc/4.0/ *
dc.subject pathobiology canine osteosarcoma appendicular en_US
dc.title Genomic Analysis of Canine Appendicular Osteosarcoma Metastases en_US
dc.type Thesis en_US
dc.degree.programme Pathobiology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Pathobiology en_US
dc.description.embargo 2020-12-09
dc.rights.license All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.


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Attribution-NonCommercial 4.0 International Except where otherwise noted, this item's license is described as Attribution-NonCommercial 4.0 International