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Development of Rapid Propagation Systems for Hemerocallis sp. (Daylilies)

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Title: Development of Rapid Propagation Systems for Hemerocallis sp. (Daylilies)
Author: Callaghan, Joshua; Jones, Max
Department: Department of Plant Agriculture
Program: Plant Agriculture
Advisor: Jones, Max
Abstract: Daylilies (Hemerocallis spp.) are ornamental flowering plants with over 80,000 unique registered cultivars. Traditionally propagated through division, multiplication rates range from 1-20 divisions per season depending on cultivar, leading to a delay of up to 17 years for newly registered cultivars to reach a marketable level of plant material. Micropropagation offers an alternative approach that can dramatically increase multiplication rates and is used extensively for the species. However, protocols have only been developed for a selected number of genotypes and many protocols go through a callus phase introducing concern over somaclonal variation. The objective of this study was to evaluate the multiplication stage of micropropagation to optimize a protocol that minimizes unwanted callus with some degree of compatibility among different cultivars. With the use of multiple cytokinins in a liquid medium, a protocol has been established and successfully used to bring several cultivars into culture.
URI: http://hdl.handle.net/10214/17751
Date: 2020-01-06
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