Title:
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Development of Rapid Propagation Systems for Hemerocallis sp. (Daylilies) |
Author:
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Callaghan, Joshua; Jones, Max
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Department:
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Department of Plant Agriculture |
Program:
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Plant Agriculture |
Advisor:
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Jones, Max |
Abstract:
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Daylilies (Hemerocallis spp.) are ornamental flowering plants with over 80,000 unique registered cultivars. Traditionally propagated through division, multiplication rates range from 1-20 divisions per season depending on cultivar, leading to a delay of up to 17 years for newly registered cultivars to reach a marketable level of plant material. Micropropagation offers an alternative approach that can dramatically increase multiplication rates and is used extensively for the species. However, protocols have only been developed for a selected number of genotypes and many protocols go through a callus phase introducing concern over somaclonal variation. The objective of this study was to evaluate the multiplication stage of micropropagation to optimize a protocol that minimizes unwanted callus with some degree of compatibility among different cultivars. With the use of multiple cytokinins in a liquid medium, a protocol has been established and successfully used to bring several cultivars into culture. |
URI:
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http://hdl.handle.net/10214/17751
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Date:
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2020-01-06 |
Terms of Use:
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