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1H-Nuclear Magnetic Resonance Metabolomics Study of Perfluoroalkyl Substance Exposure on the Metabolic Profile of Arabidopsis thaliana

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dc.contributor.advisor Longstaffe, James
dc.contributor.author O'Hara, Liam
dc.date.accessioned 2019-12-20T15:00:22Z
dc.date.available 2019-12-20T15:00:22Z
dc.date.copyright 2019-12
dc.date.created 2019-12-05
dc.date.issued 2019-12-20
dc.identifier.uri http://hdl.handle.net/10214/17672
dc.description.abstract This thesis investigates the use of proton (1H) nuclear magnetic resonance (NMR) spectroscopy for non-targeted metabolomics to measure the metabolic response of Arabidopsis thaliana exposed to PFAS. The metabolic response of model plant species A. thaliana exposed to the common PFAS compounds perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) is observed using 1H-NMR-based non-targeted metabolomics. Polar metabolites are extracted from the leaf tissue of A. thaliana using a biphasic extraction and analyzed using 1H-NMR, followed by multivariate statistical analysis using principal component analysis (PCA) to determine the changes in the metabolic profile efficiently and reproducibility. Exposure to high concentrations of PFAS results in changes in the physiological appearance and large changes in metabolic profile. At lower, more environmentally relevant concentrations, exposure to PFAS results in no change in the physiological state of A. thaliana though changes are observed in the metabolic profile. en_US
dc.description.sponsorship Geosyntec Consultants International en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject metabolomics en_US
dc.subject nuclear magnetic resonance en_US
dc.subject arabidopsis thaliana en_US
dc.subject perfluorooctanoic acid en_US
dc.subject perfluorooctane sulfonic acid en_US
dc.subject non-targeted en_US
dc.title 1H-Nuclear Magnetic Resonance Metabolomics Study of Perfluoroalkyl Substance Exposure on the Metabolic Profile of Arabidopsis thaliana en_US
dc.type Thesis en_US
dc.degree.programme Environmental Sciences en_US
dc.degree.name Master of Science en_US
dc.degree.department School of Environmental Sciences en_US
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