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Development of a Full-Length Infectious Clone of Grapevine rupestris stem pitting-associated virus strain Syrah and GFP-Tagged and VIGS Vectors for Vitis vinifera

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dc.contributor.advisor Meng, Baozhong
dc.contributor.author Roscow, Olivia
dc.date.accessioned 2019-12-16T21:33:23Z
dc.date.available 2019-12-16T21:33:23Z
dc.date.copyright 2019-12
dc.date.created 2019-11-27
dc.date.issued 2019-12-16
dc.identifier.uri http://hdl.handle.net/10214/17655
dc.description.abstract Grapevines are an important Canadian crop, yet quality, health, and yield are threatened by viral infections and coinfections with multiple viruses complicate disease associations. Grapevine rupestris stem pitting-associated virus (GRSPaV) is a single-stranded, positive-sense RNA virus that is widely distributed worldwide and is associated with Rupestris Stem Pitting, Syrah Decline, and Grapevine Vein Necrosis. Specifically the SY strain is associated with unhealthy vines, possibly being more pathogenic. An infectious clone can help clarify disease associations and viral biology by reproducing singular infections in virus-free plants. MGT, a clone related to SY, was constructed and a GFP-tagged version produced GFP faster and to a greater degree than the asymptomatic GG strain in Nicotiana benthamiana, as determined by Western blotting and epifluorescence microscopy. A Virus-Induced Gene Silencing (VIGS) clone was also constructed for future study of grapevine genomics. These N. benthamiana experiments provide a foundation for continued work with this clone with grapevines. en_US
dc.description.sponsorship NSERC, Province of Ontario, University of Guelph en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-ShareAlike 4.0 International *
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/4.0/ *
dc.subject grapevine en_US
dc.subject rupestris en_US
dc.subject stem en_US
dc.subject pitting en_US
dc.subject virus en_US
dc.subject virology en_US
dc.subject pseudoknot en_US
dc.subject plant en_US
dc.subject vigs en_US
dc.subject vitis en_US
dc.subject syrah en_US
dc.subject cdna en_US
dc.subject clone en_US
dc.title Development of a Full-Length Infectious Clone of Grapevine rupestris stem pitting-associated virus strain Syrah and GFP-Tagged and VIGS Vectors for Vitis vinifera en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US
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