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Effect of Extracorporeal Shock Wave Therapy on Equine Umbilical Cord Blood Mesenchymal Stromal Cells in vitro

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dc.contributor.advisor Koenig, Judith
dc.contributor.advisor Koch, Thomas
dc.contributor.author Salcedo Jiménez, Ramés II
dc.date.accessioned 2019-12-12T20:31:17Z
dc.date.available 2019-12-12T20:31:17Z
dc.date.copyright 2019-12
dc.date.created 2019-11-18
dc.date.issued 2019-12-12
dc.identifier.uri http://hdl.handle.net/10214/17640
dc.description.abstract Extracorporeal shock wave therapy (ESWT) has been shown to induce different biological effects on a variety of cells, including regulation and stimulation of their function and metabolism. ESWT can promote different biological responses such as proliferation, migration, and regenerations of cells. Recent studies have shown that mesenchymal stromal cells (MSCs) secrete factors that enhance regeneration of tissues, stimulate proliferation and differentiation of cells and decrease inflammatory and immune-reactions. Clinically, the combination of these two therapies has been used as treatment for tendon and ligament lesions in horses; however, there is no scientific evidence supporting this combination of therapies in vivo. Therefore, the objectives of the study were to evaluate if ESWT affects equine umbilical cord blood mesenchymal stromal cells (CB-MSCs) proliferative, metabolic, migrative, differentiation, and immunomodulatory properties in vitro. Equine CB-MSCs were treated using an electrohydraulic shock wave generator attached to a water bath. All experiments were performed as triplicates. Proliferation, viability, migration and immunomodulatory properties of the cells were evaluated. Equine CB-MSCs were induced to evaluate their trilineage differentiation potential. ESWT treated cells had increased metabolic activity, showed positive adipogenic, osteogenic, and chondrogenic differentiation, and showed higher potential for differentiation towards the adipogenic and osteogenic cell fates. ESWT treated cells showed similar immunomodulatory properties to non-ESWT treated cells. Equine CB-MSCs are sensitive to ESWT treatment and showed increased metabolic, adipogenic and osteogenic activity, but unaltered immunosuppressive properties. In vivo studies are warranted to determine if synergistic effects can be obtained in the treatment musculoskeletal injuries if ESWT and equine CB-MSC therapies are combined. en_US
dc.description.sponsorship Equine Guelph, CONACYT en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject Extracorporeal shock wave therapy en_US
dc.subject Shock wave en_US
dc.subject Mesenchymal Stromal Cells en_US
dc.subject Cord blood en_US
dc.subject Equine en_US
dc.subject Stem cells en_US
dc.title Effect of Extracorporeal Shock Wave Therapy on Equine Umbilical Cord Blood Mesenchymal Stromal Cells in vitro en_US
dc.type Thesis en_US
dc.degree.programme Veterinary Science en_US
dc.degree.name Doctor of Veterinary Science en_US
dc.degree.department Department of Clinical Studies en_US
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Attribution-NonCommercial-NoDerivatives 4.0 International Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International