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The Anatomical Distribution of Mechanoreceptors across the Hind Paw of Integrin a1-null and Wild Type Mice using Serial Histological Sectioning

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dc.contributor.advisor Clark, Andrea
dc.contributor.author Wai, Valerie
dc.date.accessioned 2019-10-11T18:28:15Z
dc.date.copyright 2019-09-01
dc.date.created 2019-09-23
dc.date.issued 2019-10-11
dc.identifier.uri http://hdl.handle.net/10214/17509
dc.description.abstract Electrophysiological studies have mapped afferent responses to mechanical stimuli of the mouse hind paw skin, however the anatomical distribution of mechanoreceptors is unknown. The purpose of this thesis was to develop a protocol for serial histological sectioning of the intact mouse hind paw to quantify mechanoreceptors. Hind paw dissection, tissue preparation, chemical processing, sectioning and staining were successfully optimized. These methods were then applied to determine the anatomical distribution of mechanoreceptors across the hind paws of itga1-null and wild type mice. Meissner’s corpuscles were located exclusively in the glabrous footpad and tip of the digit skin while Merkel cells were found throughout the hind paw. Ruffini endings and Pacinian corpuscles were absent. The number of Merkel cells in itga1-null mice was four times less than controls, and itga1-null mice were less sensitive to static skin indentation suggesting an important role for integrin α1β1 in mechanosensation of the hind paw. en_US
dc.description.sponsorship NSERC Discovery Grant (A.C.) en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject mechanoreceptors en_US
dc.subject histology en_US
dc.subject mouse hind paw en_US
dc.subject serial sectioning en_US
dc.subject decalcification en_US
dc.subject Meissner's corpuscles en_US
dc.subject Merkel cells en_US
dc.title The Anatomical Distribution of Mechanoreceptors across the Hind Paw of Integrin a1-null and Wild Type Mice using Serial Histological Sectioning en_US
dc.type Thesis en_US
dc.degree.programme Human Health and Nutritional Sciences en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Human Health and Nutritional Sciences en_US
dc.description.embargo 2020-09-26
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