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In Vitro Propagation and Cryopreservation of American Chestnut (Castanea dentata (Marsh.) Borkh.)

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dc.contributor.advisor Saxena, Praveen Liu, Zhuoya 2019-10-03T16:01:24Z 2019-10-03T16:01:24Z 2019-09 2019-09-23 2019-10-03
dc.description.abstract American chestnut (Castanea dentata) is an endangered species in Canada. The population of American chestnut trees declined significantly due to the invasion of a fungal pathogen causing chestnut blight. American chestnut requires efficient strategies for propagation and preservation for species recovery. An in-vitro technology using liquid-based temporary immersion system (TIS) was developed for micropropagation of American chestnut. Optimum shoot growth was obtained in DKW (Driver and Kuniyuki Walnut) medium supplemented with 2.2 μM 6-benzylaminopurine and 1.0 μM gibberellic acid. Maximum microshoots developed roots on medium comprised 15.0 μM 3-Indolebutyric acid. A cryopreservation protocol was developed through droplet vitrification of shoots for germplasm preservation. Optimal regeneration of shoot tips was achieved by preculturing explants on stepwise concentrations of sucrose and subsequent dehydration in PVS3 for 30 min. These protocols may facilitate propagation and long-term preservation of American chestnut and possibly other woody plant species. en_US
dc.language.iso en en_US
dc.subject In Vitro Propagation en_US
dc.subject Micropropagation en_US
dc.subject Cryopreservation en_US
dc.subject American Chestnut en_US
dc.title In Vitro Propagation and Cryopreservation of American Chestnut (Castanea dentata (Marsh.) Borkh.) en_US
dc.type Thesis en_US Plant Agriculture en_US Master of Science en_US Department of Plant Agriculture en_US
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