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Investigation of the role of Naked 1 in Wnt Signaling

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dc.contributor.advisor Van Raay, Terry
dc.contributor.author Khan, Haider
dc.contributor.author Van Raay, Terry
dc.date.accessioned 2019-09-20T17:21:29Z
dc.date.available 2019-09-20T17:21:29Z
dc.date.copyright 2019-09
dc.date.created 2019-09-11
dc.date.issued 2019-09-20
dc.identifier.uri http://hdl.handle.net/10214/17480
dc.description.abstract Naked Cuticle 1 (Nkd1) is an obligate Wnt signaling target gene that participates in a negative feedback loop to antagonize Wnt signals. Previous research has shown that Nkd1 interacts with cytoplasmic B-catenin to attenuate the pathway, presumably in cases where the pathway has been over-activated. To validate previous findings, we generated complete nkd1 knockout zebrafish using CRISPR/cas9 and determined that loss of Nkd1 has no phenotypic consequence in zebrafish embryos. The effect of nkd1 loss of function is seen at the molecular level, where qRT-PCR analysis revealed that in cases of excess Wnt signaling, nkd1 -/- mutant embryos had higher expression of Wnt target gene sp5 relative to WT. Immunohistochemistry also revealed that in cases of excess Wnt signaling, nkd1 -/- mutant embryos had higher levels of nuclear B-catenin relative to WT. Based on our findings, we determined that loss of Nkd1 hypersensitizes embryos to Wnt signaling. en_US
dc.language.iso en en_US
dc.subject Wnt en_US
dc.subject signaling en_US
dc.subject negative feedback en_US
dc.subject regulation en_US
dc.subject naked 1 en_US
dc.subject nkd 1 en_US
dc.subject beta catenin en_US
dc.title Investigation of the role of Naked 1 in Wnt Signaling en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
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