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Investigation of the role of Naked 1 in Wnt Signaling

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Title: Investigation of the role of Naked 1 in Wnt Signaling
Author: Khan, Haider; Van Raay, Terry
Program: Molecular and Cellular Biology
Advisor: Van Raay, Terry
Abstract: Naked Cuticle 1 (Nkd1) is an obligate Wnt signaling target gene that participates in a negative feedback loop to antagonize Wnt signals. Previous research has shown that Nkd1 interacts with cytoplasmic B-catenin to attenuate the pathway, presumably in cases where the pathway has been over-activated. To validate previous findings, we generated complete nkd1 knockout zebrafish using CRISPR/cas9 and determined that loss of Nkd1 has no phenotypic consequence in zebrafish embryos. The effect of nkd1 loss of function is seen at the molecular level, where qRT-PCR analysis revealed that in cases of excess Wnt signaling, nkd1 -/- mutant embryos had higher expression of Wnt target gene sp5 relative to WT. Immunohistochemistry also revealed that in cases of excess Wnt signaling, nkd1 -/- mutant embryos had higher levels of nuclear B-catenin relative to WT. Based on our findings, we determined that loss of Nkd1 hypersensitizes embryos to Wnt signaling.
URI: http://hdl.handle.net/10214/17480
Date: 2019-09
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