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Frequency and characterization of extended-spectrum cephalosporin- and gentamicin-resistant Enterobacterales from Canadian turkeys and distribution of associated resistance determinants

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Title: Frequency and characterization of extended-spectrum cephalosporin- and gentamicin-resistant Enterobacterales from Canadian turkeys and distribution of associated resistance determinants
Author: Moffat, Jonathan
Department: Department of Pathobiology
Program: Pathobiology
Advisor: Boerlin, Patrick
Abstract: Frequency of gentamicin- and extended-spectrum cephalosporin (ESC)-resistant Enterobacterales in turkey fecal samples following enrichment or direct plating, and from diagnostic isolates, were determined. Gentamicin- and ESC-resistant Enterobacteriales were recovered from 100% and 81% of farms sampled using selective enrichments, respectively. Seventy-one percent of ESC resistant E. coli were blaCMY-2-positive. Whole genome sequencing of blaCTX-M isolates showed multiple variants of this gene present in a diversity of Achtman sequence types, with blaCTX-M-1 and blaCTX-M-55 being the most common. The most frequent gentamicin resistance gene was aac(3)-VI and was often found in isolates with genes encoding resistance to sulphonamides and tetracyclines, which are antimicrobials commonly used in the treatment of turkey diseases. ESC- and gentamicin-resistant strains are widespread and are often multi-drug resistant, but make up the minority of the E. coli population. Therefore, careful selection of substitute antimicrobials or alternatives to antimicrobials are needed to avoid spread of these resistant strains.
URI: http://hdl.handle.net/10214/17465
Date: 2019-09
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