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3D printing for the development and evaluation of three novel liquid media systems for plant tissue culture

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dc.contributor.advisor Jones, Max
dc.contributor.author Piunno, Kevin
dc.date.accessioned 2019-09-12T18:58:03Z
dc.date.available 2019-09-12T18:58:03Z
dc.date.copyright 2019-09-05
dc.date.created 2019-09-05
dc.date.issued 2019-09-12
dc.identifier.uri http://hdl.handle.net/10214/17462
dc.description.abstract Labour represents a significant portion of the cost for micropropagated plants, and automation is a potential solution to increase efficiency. Micropropagation most often uses semisolid media, but many authors suggest that liquid systems are the key to developing efficient automated systems. However, liquid cultures require ancillary pneumatic or mechanical systems to prevent hypoxia or hyperhydricity of tissues. In this study, three novel liquid culture systems were designed, produced, and evaluated. The design and production of these systems was enabled using low-cost consumer-grade 3D printing technology. The first system uses hydrostatics to enable passive thin-media layer liquid culture. The second uses a two-pieces scaffold to facilitate rooting in liquid media. The third device overcomes the main disadvantages of mechanical temporary immersion systems by allowing full immersion timing control and a small footprint. Together, these novel approaches to liquid culture offer new choices to industry and may help move toward increased automation. en_US
dc.language.iso en en_US
dc.subject plant tissue culture en_US
dc.subject liquid media en_US
dc.subject 3d printing en_US
dc.title 3D printing for the development and evaluation of three novel liquid media systems for plant tissue culture en_US
dc.type Thesis en_US
dc.degree.programme Plant Agriculture en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Plant Agriculture en_US
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