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The Utility of Immune Profiling on Cerebrospinal Fluid to Diagnose Central Nervous System Diseases in Dogs

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dc.contributor.advisor Beeler-Marfisi, Janet
dc.contributor.advisor Keller, Stefan
dc.contributor.advisor James, Fiona
dc.contributor.author Morrill, Tamara
dc.date.accessioned 2019-09-11T15:17:17Z
dc.date.copyright 2019-09
dc.date.created 2019-09-04
dc.date.issued 2019-09-11
dc.identifier.uri http://hdl.handle.net/10214/17425
dc.description.abstract Central nervous system (CNS) diseases in dogs can be challenging to diagnose due to non-specific clinical findings. Cerebrospinal fluid (CSF) is routinely collected as part of the neurological work-up. Our long-term goal is to use immune profiling, i.e. lymphocyte antigen receptor repertoire sequencing, as a novel method to diagnose canine CNS diseases based on disease-specific ‘immune signatures’ in CSF. Our hypothesis is that etiologically distinct diseases are associated with specific immune repertoires. The objectives of this study were 1) to identify technical determinants for successful immune profiling and 2) to generate preliminary immune repertoire data from CSF of dogs with neurological disease. Our results suggest that LAR genes can be sequenced from both, cell-associated and cell-free DNA, that lymphocyte abundance is a predictor of sequencing success and that whole genome amplification is of no benefit. Finally, this study generated the first immune repertoire data for inflammatory CNS diseases in dogs. en_US
dc.description.sponsorship Pet Trust grant #053652, NSERC Discovery grant #401122, OVC Pet Trust Scholar MSc scholarship en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-ShareAlike 4.0 International *
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/4.0/ *
dc.subject Cerebrospinal fluid en_US
dc.subject Canine en_US
dc.subject Central nervous system diseases en_US
dc.subject Next generation sequencing en_US
dc.subject Immune profiling en_US
dc.subject Lymphocyte antigen receptor en_US
dc.subject Veterinary diagnostics en_US
dc.title The Utility of Immune Profiling on Cerebrospinal Fluid to Diagnose Central Nervous System Diseases in Dogs en_US
dc.type Thesis en_US
dc.degree.programme Pathobiology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Pathobiology en_US
dc.description.embargo 2020-09-11
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Attribution-NonCommercial-ShareAlike 4.0 International Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International