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The Utility of Immune Profiling on Cerebrospinal Fluid to Diagnose Central Nervous System Diseases in Dogs

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Title: The Utility of Immune Profiling on Cerebrospinal Fluid to Diagnose Central Nervous System Diseases in Dogs
Author: Morrill, Tamara
Department: Department of Pathobiology
Program: Pathobiology
Advisor: Beeler-Marfisi, JanetKeller, StefanJames, Fiona
Abstract: Central nervous system (CNS) diseases in dogs can be challenging to diagnose due to non-specific clinical findings. Cerebrospinal fluid (CSF) is routinely collected as part of the neurological work-up. Our long-term goal is to use immune profiling, i.e. lymphocyte antigen receptor repertoire sequencing, as a novel method to diagnose canine CNS diseases based on disease-specific ‘immune signatures’ in CSF. Our hypothesis is that etiologically distinct diseases are associated with specific immune repertoires. The objectives of this study were 1) to identify technical determinants for successful immune profiling and 2) to generate preliminary immune repertoire data from CSF of dogs with neurological disease. Our results suggest that LAR genes can be sequenced from both, cell-associated and cell-free DNA, that lymphocyte abundance is a predictor of sequencing success and that whole genome amplification is of no benefit. Finally, this study generated the first immune repertoire data for inflammatory CNS diseases in dogs.
URI: http://hdl.handle.net/10214/17425
Date: 2019-09
Rights: Attribution-NonCommercial-ShareAlike 4.0 International
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Attribution-NonCommercial-ShareAlike 4.0 International Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International