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Plasma microRNA Profile in Canine Appendicular Osteosarcoma Patients

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Title: Plasma microRNA Profile in Canine Appendicular Osteosarcoma Patients
Author: Edson, Michael
Department: Department of Pathobiology
Program: Pathobiology
Advisor: Wood, Geoffrey
Abstract: Osteosarcoma (OSA) is the most common primary bone tumor in dogs. The standard of care for canine appendicular OSA involves amputation followed by chemotherapy. Currently, there are no established biomarkers to determine which dogs benefit the most from chemotherapy. MicroRNAs (miRNAs) are small non-coding RNAs that are potential biomarkers of osteosarcoma. Plasma miRNA levels were profiled in healthy dogs and OSA patients, collected before and after amputation. Quantitative real-time PCR was conducted to determine the levels of 277 miRNAs on a miRNome PCR array. From these findings, a custom PCR array was designed and used to examine miRNA levels and correlations to clinical outcome in 20 control and 28 OSA cases. Additionally, miRNA levels in erythrocytes were analyzed. Overall, 37 plasma miRNAs and 1 erythrocyte miRNA had significant level changes. Furthermore, 24 plasma miRNAs were correlated to clinical outcome. These miRNAs could improve decision making in treatments for OSA patients.
URI: http://hdl.handle.net/10214/17393
Date: 2019-09
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