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Glucocorticoid Regulation of the G-protein Coupled Estrogen Receptor (GPER) in Mouse Hippocampal Neurons

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Title: Glucocorticoid Regulation of the G-protein Coupled Estrogen Receptor (GPER) in Mouse Hippocampal Neurons
Author: Nicholson, Kate
Department: Department of Biomedical Sciences
Program: Biomedical Sciences
Advisor: MacLusky, Neil
Abstract: The most prevalent estrogen, 17β-estradiol, binds the non-classical G-protein coupled estrogen receptor (GPER) with high affinity resulting in rapid activation of the c-jun N terminal kinase (JNK) pathway. GPER activation mediates some of the rapid neurotrophic and memory-enhancing effects of 17β-estradiol in the female hippocampus. However, exposure to stressful stimuli may impair these beneficial effects. This thesis characterizes neurosteroid receptor expression in murine-derived mHippoE cell lines that are subsequently used to investigate the glucocorticoid regulation of GPER protein expression and functional activation. This thesis demonstrates that 24-hour treatment with a glucocorticoid receptor agonist reduces GPER protein expression and activation of JNK in female-derived mHippoE-14s. Using an in vivo model, treatment with glucocorticoids significantly reduces hippocampal activation of JNK in female ovariectomized CD1 mice. Collectively, this thesis uses in vitro and in vivo models to characterize glucocorticoid regulation of GPER expression and signalling in female murine hippocampal neurons.
URI: http://hdl.handle.net/10214/17311
Date: 2019-08
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