Main content

The protective actions of the cardiac CRF system in rainbow trout (Oncorhynchus mykiss) in response to hypoxia and reoxygenation

Show full item record

Title: The protective actions of the cardiac CRF system in rainbow trout (Oncorhynchus mykiss) in response to hypoxia and reoxygenation
Author: Freedman, Calli
Department: Department of Integrative Biology
Program: Integrative Biology
Advisor: Bernier, NicholasGillis, Todd
Abstract: The corticotropin-releasing factor (CRF) system has been shown to exert physiological actions in the cardiovascular system of mammals, including positive inotropy and cardioprotection against ischemia-reperfusion injury. In contrast, little is known about the cardiac CRF system in non-mammalian species. I investigated the role of CRF in the cardiac response to acute hypoxia in rainbow trout. I examined myocardial contractility during hypoxia/reoxygenation by measuring ventricular pressure generation of ex vivo Langendorff-perfused hearts and investigated the effects of pre-treatment with 1.0 nM trout CRF or 10 nM antalarmin, a CRF type 1 receptor antagonist. All CRF system components are expressed in the heart where the dominant receptor type is CRF-2b and the dominant ligand is CRFa1. Antalarmin exerted significant negative inotropic and lusitropic effects throughout the hypoxia exposure and recovery periods. These results suggest that endogenous CRF plays a cardioprotective role during hypoxia/reoxygenation and is involved in the maintenance/recovery of cardiac function.
URI: http://hdl.handle.net/10214/16986
Date: 2019-08
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
Terms of Use: All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.


Files in this item

Files Size Format View Description
Freedman_Calli_201908_MSc.pdfuntranslated 877.7Kb PDF View/Open Main thesis

This item appears in the following Collection(s)

Show full item record

Attribution-NonCommercial-NoDerivatives 4.0 International Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International