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Differential DNA Methylation of Seed Yield QTL in Intercontinental Mega-Environments in a Canadian x Chinese Soybean Population

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Title: Differential DNA Methylation of Seed Yield QTL in Intercontinental Mega-Environments in a Canadian x Chinese Soybean Population
Author: Templeman, Colbey Jane
Department: Department of Plant Agriculture
Program: Plant Agriculture
Advisor: Rajcan, Istvan
Abstract: DNA methylation is an epigenetic modification that can produce phenotypic changes that impact a plant’s adaptation to the environment. Sodium bisulfite sequencing was carried out on the genomic regions associated with seed yield in multiple (QTLU) and specific (QTLSP) mega-environments (ME). One QTLU (Satt139) and one QTLSP (Satt063) were evaluated on source seed grown in three MEs (Canada, USA, and China in 2010) of four selected RILs (28, 47, 4 and 90) derived from OAC Millennium x Heinong 38, and the parents. The source seed was grown in two field locations (Woodstock and Elora, ON) in both 2012 and 2013. Demethylation was observed in RIL 47 (China) in the Satt063 (QTLSP) region when grown in Elora and Woodstock in 2013. Due to methodology limitations, further research on DNA methylation is warranted to determine its role in the expression of yield QTL across different ME.
URI: http://hdl.handle.net/10214/16964
Date: 2019-08
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