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Effects of Branched Chain Amino Acid Transaminase 1 in Claudin-low Breast Cancer

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Title: Effects of Branched Chain Amino Acid Transaminase 1 in Claudin-low Breast Cancer
Author: Reynen, Lisa
Department: Department of Biomedical Sciences
Program: Biomedical Sciences
Advisor: Moorehead, Roger
Abstract: Breast cancer can be classified into five distinct subtypes. One subtype, claudin-low breast cancer, accounts for approximately 7% of the breast cancer cases and these tumors are notoriously aggressive. RNA sequencing of human claudin-low breast cancers by other groups and RNA sequencing of a murine claudin-low breast cancer cell line by our group has revealed that Bcat1 is up-regulated in this breast cancer subtype. Bcat1 regulates the metabolism of branched chain amino acids. Based on this data we hypothesized that the expression of Bcat1 is driving the aggressive nature of this cancer subtype. BCAT1 was confirmed to be up-regulated in our murine claudin-low breast cancer cell line at the mRNA and protein level. However, our study demonstrates that BCAT1 does not influence proliferation, survival, or migration. However, it does appear that the reverse BCAT1 reaction may be favoured in our murine claudin-low breast cancer cell line.
URI: http://hdl.handle.net/10214/16953
Date: 2019-08
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