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The Creation of a Phytoglycogen-Functionalized SPR Sensor Surface and the Binding of Concanavalin A to Phytoglycogen

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dc.contributor.advisor Dutcher, John
dc.contributor.author Charlesworth, Kathleen
dc.date.accessioned 2019-08-21T17:41:48Z
dc.date.available 2019-08-21T17:41:48Z
dc.date.copyright 2019-08
dc.date.created 2019-08-16
dc.date.issued 2019-08-21
dc.identifier.uri http://hdl.handle.net/10214/16929
dc.description.abstract Phytoglycogen is a glucose polymer that occurs naturally in the form of highly branched, compact nanoparticles. Because of their tree-like or dendrimeric structure, phytoglycogen nanoparticles have unique properties, such as a strong interaction with water, which makes them attractive for use in applications ranging from cosmetics to drug delivery. Many of these applications rely on the binding of small molecules onto phytoglycogen nanoparticles. Surface Plasmon Resonance (SPR) is a sensitive experimental technique, based on the resonant absorption of light within an ultrathin gold film, that can be used to measure the binding kinetics and affinities of small molecules. We have successfully created a stable phytoglycogen-functionalized gold surface, using 4-Mercaptophenylboronic Acid as a linker between the gold layer and phytoglycogen. This has allowed us to use SPR to measure the association constant between phytoglycogen and Concanavalin A to be 2.87±0.44×10^5 M-1 using the Langmuir adsorption model fit. en_US
dc.description.sponsorship NSERC CGS-M en_US
dc.language.iso en en_US
dc.subject physics en_US
dc.subject biophysics en_US
dc.subject surface plasmon resonance en_US
dc.subject concanavalin A en_US
dc.subject phytoglycogen en_US
dc.subject association constant en_US
dc.subject polymer en_US
dc.subject bioactive en_US
dc.title The Creation of a Phytoglycogen-Functionalized SPR Sensor Surface and the Binding of Concanavalin A to Phytoglycogen en_US
dc.type Thesis en_US
dc.degree.programme Physics en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Physics en_US
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