Main content

A Polysaccharide-Based Vaccine Against Campylobacter jejuni

Show full item record

Title: A Polysaccharide-Based Vaccine Against Campylobacter jejuni
Author: Crha, Jennifer
Department: Department of Chemistry
Program: Chemistry
Advisor: Monteiro, Mario
Abstract: This thesis describes the development of a polysaccharide-based vaccine against the major cause of bacterial diarrhea, the food-borne pathogen, Campylobacter jejuni. C. jejuni is a leading cause of traveler’s diarrhea and can be deadly, especially in children. Military forces are a group that is highly prone to Campylobacteriosis. Weeks after a diarrheal episode, a patient may also suffer from autoimmune diseases, such as Guillain-Barré Syndrome. C. jejuni exposes serotype-specific polysaccharide capsules (CPS) that play a role in virulence. In our group, we study the use of CPSs as C. jejuni vaccine targets, and previously an efficacious prototype CPS conjugate vaccine against C. jejuni serotype HS:23 was synthesized. In this work, fine structural analysis of serotype HS:4B (strain PG3643) CPS revealed a disaccharide repeating unit composed of glucopyranose (Glc) and L-glycero-D-ido-heptopyranose (Hep), or its 6-deoxy derivative (6d-Hep): [→4)-β-ᴅ-Glc-(1→3)-β-6dHep/Hep-(1→]. This CPS was further decorated by O-methyl-phosphoramidate (MeOPN) units at positions 2 or 7 of 6d-Hep. The HS:4B CPS was selectively linked to a protein carrier that yielded a conjugate which reacted with anti-HS:4 antisera. Of particular significance, immunochemical analysis of the HS:4B conjugate also revealed that the O-methyl group in MeOPN may not be involved in antibody recognition.
URI: http://hdl.handle.net/10214/16848
Date: 2019-07
Terms of Use: All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.


Files in this item

Files Size Format View Description
Crha_Jennifer_201907_MSc.pdfuntranslated 2.231Mb PDF View/Open Full thesis

This item appears in the following Collection(s)

Show full item record