Title:
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Synthesis and Electrochemical Characterization of 4-Thio Pseudo-Glycolipids as Candidate Tethers for Lipid Bilayer Models. |
Author:
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Priske, Gillian; Su, ZhangFei; Abbasi, Fatemeh; Lipkowski, Jacek; Auzanneau, France-Isabelle
|
Department:
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Department of Chemistry |
Abstract:
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Two 4-thio pseudo-glycolipids, a disulfide and a thiol, were synthesized as candidates to build tethered
bilayer lipid membranes (tBLMs) on gold (111) surface. Monolayers of the disulfide and thiol were built
on gold surfaces using the self-assembly and Langmuir-Blodgett (LB) transfer methods. Monolayers were
prepared in several solvents and at various temperatures; their quality was assessed by differential
capacitance. Best monolayers were achieved when the disulfide was self-assembled in ethanol. The
quality of such monolayers was further improved by allowing the assembly to proceed under stirring of
the solution at increased temperatures. The charge number per adsorbed molecule and surface concentration
of disulfide on gold (111) surface were determined by chronocoulometry. A DPhPC/disulfide
tBLM was then built by vesicle fusion of 1,2-diphytanyl-sn-glycero-3-phosphocholine (DPhPC) on top of
the disulfide monolayer. The minimum capacitance of the gold electrode with tBLM (1.3 mF cm 2) was
close to the value of a real cell membrane and the AFM force spectroscopy measurements showed that
the DPhPC/disulfide tBLM had a thickness of 6.2 ± 0.6 nm consistent with the thickness expected for a
bilayer. Finally, the potential of the tBLM to study transmembrane proteins was assessed by investigating
the reconstitution of gramicidin A into the membrane by polarization modulation infrared absorption
spectroscopy (PM-IRRAS). These results demonstrate that the disulfide is a good candidate to construct
tBLMs on gold surface and to study transmembrane proteins. |
URI:
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http://hdl.handle.net/10214/16286
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Date:
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2018-12 |
Rights:
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Attribution-NonCommercial-NoDerivatives 4.0 International |
Terms of Use:
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All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated. |
Related Publications:
|
https://doi.org/10.1016/j.electacta.2018.12.048 |