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Investigating the Interaction between lytic transglycosylases and Ivyp1 & Ivyp2 in Pseudomonas aeruginosa

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Title: Investigating the Interaction between lytic transglycosylases and Ivyp1 & Ivyp2 in Pseudomonas aeruginosa
Author: Chun, Joshua
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Clarke, Anthony
Abstract: The existence of the proteinaceous inhibitors of lysozyme, Ivy, has remained a paradoxical puzzle since their discovery. From the time they were shown to inhibit MltB, efforts have been made to map the interaction of Ivyp1 and Ivyp2 with all eleven lytic transglycosylases (LTs) produced by Pseudomonas aeruginosa. Herein, this study continues to explore the hypothesis that the Ivys function as inhibitors of LTs. In doing so, a complete pH profile has been established for all LTs along with inhibition profiles by both Ivyp1 and Ivyp2 for each LT. The pH profiles reveal that the LTs operate under a slightly acidic range of pH optima. Additionally, the first experimental evidence for interactions of Ivyp1 and Ivyp2 with the LTs MltD, MltG, Slt70 and SltB1 are reported here. Altogether, these results support the hypothesis that Ivy acts to inhibit certain LTs and present possibilities for interplay with other enzymes such as PBPs.
URI: http://hdl.handle.net/10214/16105
Date: 2019-04


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